SFRP4 antibody (AA 265-346)

Catalog No. ABIN7436165

Product Details anti-SFRP4 Antibody

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Binding Specificity: AA 265-346

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)

Specificity: The antibody is a rabbit polyclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Secreted Frizzled Related Protein 4 (SFRP4) corresdonding to Met265~Val346 (Accession # Q6FHJ7)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:250-1000 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SFRP4

Target: SFRP4 (Secreted Frizzled-Related Protein 4 (SFRP4))

Alternative Name: Secreted Frizzled Related Protein 4

Background: FRP-4, FRPHE, Frizzled protein, human endometrium

Pathways: WNT Signaling