ATP4A antibody (AA 786-1014)

Catalog No. ABIN7436267

Cited in 1 publication. The Rabbit Polyclonal anti-ATP4A antibody (ABIN7436267) specifically detects ATP4A in WB, IHC, IP and ICC. The antibody is reactive with Mouse samples.

Quick Overview for ATP4A antibody (AA 786-1014) (ABIN7436267)

Target: ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ATP4A Antibody

Binding Specificity: AA 786-1014

Purpose: Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)

Specificity: The antibody is a rabbit polyclonal antibody raised against ATP4a. It has been selected for its ability to recognize ATP4a in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) corresdonding to Pro786~Cys1014 (Accession # Q91WH7) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ATP4A antibody (ABIN7436267)

Daher, Ducrot, Lefebvre, Zineeddine, Ausseil, Puy, Karim: "Crosstalk between Acidosis and Iron Metabolism: Data from In Vivo Studies." in: Metabolites, Vol. 12, Issue 2, (2022) (PubMed).

Target Details for ATP4A

Target: ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))

Alternative Name: ATP4a

Background: Gastric H,K-ATPase Alpha Subunit, H(+)-K(+)-ATPase Alpha Subunit, Proton Pump, Potassium-transporting ATPase alpha chain 1

UniProt: Q91WH7

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process