WISP1 antibody (AA 216-347)

Catalog No. ABIN7441403

The Rabbit Polyclonal anti-WISP1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect WISP1 in samples from Mouse.

Quick Overview for WISP1 antibody (AA 216-347) (ABIN7441403)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-WISP1 Antibody

Binding Specificity: AA 216-347

Purpose: Polyclonal Antibody to WNT1 Inducible Signaling Pathway Protein 1 (WISP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against WISP1. It has been selected for its ability to recognize WISP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant WNT1 Inducible Signaling Pathway Protein 1 (WISP1) corresdonding to Cys216~Arg347 (Accession # O95388)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP1

Background: CCN4, WISP1c, WISP1i, WISP1tc, CCN family member 4, Wnt-1-induced secreted protein

UniProt: O54775

Pathways: WNT Signaling, Growth Factor Binding