Nephronectin antibody (AA 420-561)

Catalog No. ABIN7436477

The Rabbit Polyclonal anti-Nephronectin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Nephronectin in samples from Mouse.

Quick Overview for Nephronectin antibody (AA 420-561) (ABIN7436477)

Target: Nephronectin (NPNT)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Nephronectin Antibody

Binding Specificity: AA 420-561

Purpose: Polyclonal Antibody to Nephronectin (NPNT)

Specificity: The antibody is a rabbit polyclonal antibody raised against NPNT. It has been selected for its ability to recognize NPNT in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nephronectin (NPNT) corresdonding to His420~Cys561 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunohistochemistry: 5-20 μg/mLImmunocytochemistry: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Nephronectin

Target: Nephronectin (NPNT)

Alternative Name: NPNT

Background: EGFL6L, POEM, Preosteoblast EGF-like repeat protein with MAM domain, Protein EGFL6-like

UniProt: Q91V88