LCAT antibody (AA 290-432)

Catalog No. ABIN7438331

The Rabbit Polyclonal anti-LCAT antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect LCAT in samples from Mouse.

Quick Overview for LCAT antibody (AA 290-432) (ABIN7438331)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCAT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-LCAT Antibody

Binding Specificity: AA 290-432

Purpose: Polyclonal Antibody to Lecithin Cholesterol Acyltransferase (LCAT)

Specificity: The antibody is a rabbit polyclonal antibody raised against LCAT. It has been selected for its ability to recognize LCAT in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Lecithin Cholesterol Acyltransferase (LCAT) corresdonding to Ile290~Pro432 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000Immunohistochemistry: 5-20 μg/mL,1:25-100Immunocytochemistry: 5-20 μg/mL,1:25-100Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT

Background: Phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

UniProt: P16301

Pathways: Lipid Metabolism