BNIP3 antibody (AA 3-134)

Catalog No. ABIN7436699

This anti-BNIP3 antibody is a Rabbit Polyclonal antibody detecting BNIP3 in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for BNIP3 antibody (AA 3-134) (ABIN7436699)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-BNIP3 Antibody

Binding Specificity: AA 3-134

Purpose: Polyclonal Antibody to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)

Specificity: The antibody is a rabbit polyclonal antibody raised against BNIP3. It has been selected for its ability to recognize BNIP3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) corresdonding to Gln3~Ala134 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3

Background: NIP3

UniProt: Q9ET45

Pathways: Autophagy, Brown Fat Cell Differentiation