Aconitase 1 antibody (AA 26-243)

Catalog No. ABIN7436732

This Rabbit Polyclonal antibody specifically detects Aconitase 1 in WB, ELISA, ICC, IHC (p), IHC (fro) and FACS. It exhibits reactivity toward Human.

Quick Overview for Aconitase 1 antibody (AA 26-243) (ABIN7436732)

Target: Aconitase 1 (ACO1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: AA 26-243

Purpose: Polyclonal Antibody to Aconitase 1 (ACO1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Aconitase 1 (ACO1) corresdonding to Lys26~Ile243 (Accession # P21399)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background: IREB1, IRP1, IREBP, Iron regulatory protein 1, Iron-responsive element-binding protein 1, Cytoplasmic aconitate hydratase, Citrate hydro-lyase, Ferritin repressor protein

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis