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Nanog antibody (AA 1-305)

The Rabbit Polyclonal anti-Nanog antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect Nanog in samples from Human.
Catalog No. ABIN7443938

Quick Overview for Nanog antibody (AA 1-305) (ABIN7443938)

Target

See all Nanog (NANOG) Antibodies
Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Nanog antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • Binding Specificity

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    AA 1-305

    Purpose

    Polyclonal Antibody to NANOG Homeobox Protein (NANOG)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against NANOG. It has been selected for its ability to recognize NANOG in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant NANOG Homeobox Protein (NANOG) corresdonding to Met1~Val305 with N-terminal His and GST Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.26 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    Alternative Name

    NANOG

    Background

    Homeobox transcription factor Nanog

    UniProt

    Q9H9S0

    Pathways

    Stem Cell Maintenance
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