HIST1H2AB antibody (AA 2-130)
Quick Overview for HIST1H2AB antibody (AA 2-130) (ABIN7438658)
Target
See all HIST1H2AB AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 2-130
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Purpose
- Polyclonal Antibody to Histone Cluster 1, H2ab (HIST1H2AB)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against HIST1H2AB. It has been selected for its ability to recognize HIST1H2AB in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Mouse
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- Recombinant Histone Cluster 1, H2ab (HIST1H2AB) corresdonding to Ser2~Lys130 (Accession # P04908) with N-terminal His and GST Tag
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Isotype
- IgG
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Application Notes
- Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 24 months
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- HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))
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Alternative Name
- HIST1H2AB
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Background
- HIST1H2AE, H2AFM, H2A/m, H2A Histone Family Member M
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UniProt
- P04908
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Pathways
- Telomere Maintenance
Target
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