Keratin 25 antibody (AA 1-446)

Catalog No. ABIN7443773

This Rabbit Polyclonal antibody specifically detects Keratin 25 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for Keratin 25 antibody (AA 1-446) (ABIN7443773)

Target: Keratin 25 (KRT25)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Keratin 25 Antibody

Binding Specificity: AA 1-446

Purpose: Polyclonal Antibody to Keratin 25 (KRT25)

Specificity: The antibody is a rabbit polyclonal antibody raised against KRT25. It has been selected for its ability to recognize KRT25 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Keratin 25 (KRT25) corresdonding to Met1~Asn446

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Keratin 25

Target: Keratin 25 (KRT25)

Alternative Name: KRT25

Background: KRT25A, CK-25, K25, Cytokeratin-25, Keratin, type I cytoskeletal 25, Type I inner root sheath-specific keratin-K25irs1

UniProt: Q8VCW2