Cytokeratin 28 antibody (AA 1-462)

Catalog No. ABIN7443671

The Rabbit Polyclonal anti-Cytokeratin 28 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Cytokeratin 28 in samples from Mouse.

Quick Overview for Cytokeratin 28 antibody (AA 1-462) (ABIN7443671)

Target: Cytokeratin 28 (KRT28)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 28 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cytokeratin 28 Antibody

Binding Specificity: AA 1-462

Purpose: Polyclonal Antibody to Keratin 28 (KRT28)

Specificity: The antibody is a rabbit polyclonal antibody raised against KRT28. It has been selected for its ability to recognize KRT28 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Keratin 28 (KRT28) corresdonding to Met1~Phe462 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Cytokeratin 28

Target: Cytokeratin 28 (KRT28)

Alternative Name: KRT28

Background: KRT25D, K28, K25D, Cytokeratin-28, Type I inner root sheath-specific keratin-K25irs4, Keratin, type I cytoskeletal 28

UniProt: A6BLY7