Glutaminase antibody

Catalog No. ABIN7247984

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human GLS

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.02 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS (GLS Products)

Background: GLS,also named as GLS1 and KIAA0838,belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-,glutamate-,and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells,in accordance with measurements of glutaminase activity,and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase. It has 3 isoforms named as KGA,GAM,GAC.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 73 kDa

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect