Glutaminase antibody (AA 221-533)

Catalog No. ABIN7436597

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Binding Specificity: AA 221-533

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Glutaminase (GLS)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLS. It has been selected for its ability to recognize GLS in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glutaminase (GLS) corresdonding to Ile221~Leu533 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: Glutaminase (GLS Products)

Background: GLS1, Glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect