Glutaminase antibody (C-Term)

Catalog No. ABIN2786054

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VNPFPKDRWN NTPMDEALHF GHHDVFKILQ EYQVQYTPQG DSDNGKENQT

Predicted Reactivity: Dog: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GLS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the c terminal region of human GLS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 669 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Glutaminase antibody (ABIN2786054)

Sahai: "Glutaminase in human platelets." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 127, Issue 2, pp. 197-203, (1983) (PubMed).

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS (GLS Products)

Background: Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances
Alias Symbols: AAD20, DKFZp686O15119, FLJ10358, GLS1, KIAA0838, GAC, GAM, KGA
Protein Interaction Partner: UBC, PEPD, MVD, GNS, MYL12A, GDA, EIF5A, CAPN1, ATF2, ATXN10, CUL5, TAX1BP3, PARD6A, SNTA1,
Protein Size: 669

Molecular Weight: 73 kDa

Gene ID: 2744

NCBI Accession: NM_014905, NP_055720

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect