ALKBH8 antibody

Catalog No. ABIN7249214

The Rabbit Polyclonal anti-ALKBH8 antibody has been validated for WB, ELISA and IHC. It is suitable to detect ALKBH8 in samples from Human.

Quick Overview for ALKBH8 antibody (ABIN7249214)

Target: ALKBH8 (AlkB, Alkylation Repair Homolog 8 (ALKBH8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ALKBH8 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Full length fusion protein

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ALKBH8

Target: ALKBH8 (AlkB, Alkylation Repair Homolog 8 (ALKBH8))

Alternative Name: ALKBH8

Background: ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 75 kDa

UniProt: Q96BT7