Caspase 1 antibody

Catalog No. ABIN7249876

The Rabbit Polyclonal anti-Caspase 1 antibody has been validated for WB and IHC. It is suitable to detect Caspase 1 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 1 antibody (ABIN7249876)

Target: Caspase 1 (CASP1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Caspase 1 Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse Caspase1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:300-1:800

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: PBS with 0.02 % sodium azide, 1 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

Molecular Weight:

Observed_MW: 45-47 kDa,30 kDa,35 kDa

Calculated_MW: 45 kDa

UniProt: P29466, P29452, P43527

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome