GAPDH antibody

Catalog No. ABIN7250851

This Mouse Monoclonal antibody specifically detects GAPDH in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Pig, Dog, Monkey, Sheep, Cow, Hamster, Saccharomyces cerevisiae and Insect.

Quick Overview for GAPDH antibody (ABIN7250851)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Dog, Monkey, Sheep, Cow, Hamster, Saccharomyces cerevisiae, Insect

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 9S5

Product Details anti-GAPDH Antibody

Characteristics: Control Antibody

Purification: Protein A purification

Immunogen: Synthetic Peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. 

Molecular Weight: 37 kDa

UniProt: P04406