beta Actin antibody
-
- Target See all beta Actin (ACTB) Antibodies
- beta Actin (ACTB) (Actin, beta (ACTB))
-
Reactivity
- Human, Mouse, Rat, Chicken, Rabbit, Monkey, Sheep, Xenopus laevis
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This beta Actin antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Characteristics
- Control Antibody
- Purification
- Affinity purification
- Immunogen
- Recombinant Protein
- Isotype
- IgG
- Top Product
- Discover our top product ACTB Primary Antibody
-
-
- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze / thaw cycles.
-
- Target
- beta Actin (ACTB) (Actin, beta (ACTB))
- Alternative Name
- beta actin (ACTB Products)
- Background
- Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
- Molecular Weight
-
Observed_MW: 43 kDa
Calculated_MW: 42 kDa
- UniProt
- P60709
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
-