ERK1/2 antibody (pTyr205, pTyr222)

Catalog No. ABIN7250980

This anti-ERK1/2 antibody is a Mouse Monoclonal antibody detecting ERK1/2 in IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for ERK1/2 antibody (pTyr205, pTyr222) (ABIN7250980)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4D4

Product Details anti-ERK1/2 Antibody

Binding Specificity: pTyr205, pTyr222

Characteristics: Phosphorylated antibody

Purification: Protein A purification

Immunogen: Synthetic Peptide of Phospho-ERK 1/2 (Tyr222/205)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK 1/2

Background: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

UniProt: P27361, P28482