SETDB1 antibody

Catalog No. ABIN7253431

The Rabbit Polyclonal anti-SETDB1 antibody is suitable to detect SETDB1 in samples from Human and Mouse. It has been validated for ELISA and IHC.

Quick Overview for SETDB1 antibody (ABIN7253431)

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-SETDB1 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human SETDB1

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:300, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.08 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Alternative Name: SETDB1

Background: SETDB1,also named as ESET,KIAA0067 and KMT1E,belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1,CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions,thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation,gene silencing,and transcriptional repression. It has been identified as a target for treatment in Huntington Disease,given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis.

UniProt: Q15047