NUCB2 antibody (AA 181-280)

Catalog No. ABIN700690

This Rabbit Polyclonal antibody specifically detects NUCB2 in FACS, IF (p), IF (cc), IHC (fro) and IHC (p). It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for NUCB2 antibody (AA 181-280) (ABIN700690)

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCB2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NUCB2 Antibody

Binding Specificity: AA 181-280

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nesfatin-1/Nucb2

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NUCB2 antibody (ABIN700690)

Chen, Cao, Chang, Ma, Gao, Tong, Li, Han, Yi: "Increasing hypothalamic nucleobindin 2 levels and decreasing hypothalamic inflammation in obese male mice via diet and exercise alleviate obesity-associated hypogonadism." in: Neuropeptides, Vol. 74, pp. 34-43, (2019) (PubMed).

Jia, Li, Li, Wu, Xie, Lin: "Role of nesfatin-1 in a rat model of visceral hypersensitivity." in: World journal of gastroenterology : WJG, Vol. 19, Issue 22, pp. 3487-93, (2013) (PubMed).

Target Details for NUCB2

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Alternative Name: Nesfatin-1

Background:

Synonyms: NEFA, HEL-S-19, Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, Prepronesfatin, NUCB2

Background: Calcium-binding protein. May have a role in calcium homeostasis. Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).

Gene ID: 4925

UniProt: P80303