Histone H4 antibody (H4R3me2a)

Catalog No. ABIN7260410

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H4 antibody (H4R3me2a) (ABIN7260410)

Target: Histone H4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Histone H4 Antibody

Binding Specificity: H4R3me2a

Characteristics: Methylated antibody

Purification: Affinity purification

Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Histone H4

Target: Histone H4

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the centromeric copy.

Molecular Weight:

Observed_MW: 13 kDa

Calculated_MW: 11 kDa

Gene ID: 8370

UniProt: P62805