FIP1L1 antibody

Catalog No. ABIN7263123

This anti-FIP1L1 antibody is a Rabbit Polyclonal antibody detecting FIP1L1 in WB, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for FIP1L1 antibody (ABIN7263123)

Target: FIP1L1 (FIP1 Like 1 (FIP1L1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FIP1L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FIP1L1 Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human FIP1L1 (NP_001128409.1).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for FIP1L1

Target: FIP1L1 (FIP1 Like 1 (FIP1L1))

Alternative Name: FIP1L1

Background: This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight:

Observed_MW: 80 kDa

Calculated_MW: 40 kDa/58 kDa/65 kDa/66 kDa

Gene ID: 81608

UniProt: Q6UN15