SMC1A antibody (pSer957)

Catalog No. ABIN7265084

This anti-SMC1A antibody is a Rabbit Polyclonal antibody detecting SMC1A in WB and IF. Suitable for Human, Mouse and Rat.

Quick Overview for SMC1A antibody (pSer957) (ABIN7265084)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-SMC1A Antibody

Binding Specificity: pSer957

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: A synthetic phosphorylated peptide around S957 of human SMC1A (NP_006297.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:20-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1A

Background: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight:

Observed_MW: 160 kDa

Calculated_MW: 143 kDa

Gene ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance