GLI1 antibody (Internal Region)

Catalog No. ABIN7013859

This Goat Polyclonal antibody specifically detects GLI1 in ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for GLI1 antibody (Internal Region) (ABIN7013859)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-GLI1 Antibody

Binding Specificity: Internal Region

Purpose: GLI1

Sequence: TYSQCPRLEH YGQ

Specificity: This antibody is expected to recognize the reported isoforms (NP_005260.1, NP_001153517.1, NP_001161081.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: GLI1, GLI family zinc finger 1, GLI, glioma-associated oncogene family zinc finger 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI, zinc finger protein GLI1

Gene ID: 2735, 14632, 140589

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis