Cathepsin B antibody (Internal Region)

Catalog No. ABIN7013865

This Goat Polyclonal antibody specifically detects Cathepsin B in WB, IHC, ELISA, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Cathepsin B antibody (Internal Region) (ABIN7013865)

Target: Cathepsin B (CTSB)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cathepsin B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Cathepsin B Antibody

Binding Specificity: Internal Region

Purpose: Cathepsin B

Sequence: SDRICIHTNA HVSVE

Specificity: Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8x kDa according to NP_001899.1). Recommended concentration: 0.5-2 μg/m

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Alternative Name: CTSB

Background: CTSB, cathepsin B, APPS, CPSB, APP secretase, amyloid precursor protein secretase, cathepsin B1, cysteine protease

Gene ID: 1508

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades