EHD1 antibody (N-Term)

Catalog No. ABIN7013871

This Goat Polyclonal antibody specifically detects EHD1 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for EHD1 antibody (N-Term) (ABIN7013871)

Target: EHD1 (EH-Domain Containing 1 (EHD1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-EHD1 Antibody

Binding Specificity: N-Term

Purpose: EHD1

Sequence: FSWVSKDARR KKEPC

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 60.6 kDa according to Mouse NP_0342491) Preliminary testing also showed a band at approx 60 kDa in Human Lymph node and Rat Kidney lysate and in HeLa cell lysate. Rec

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EHD1

Target: EHD1 (EH-Domain Containing 1 (EHD1))

Alternative Name: EHD1

Background: EHD1, PAST, PAST1, H-PAST, HPAST1, EH-domain containing 1, testilin, FLJ42622, FLJ44618, OTTHUMP00000069747

Gene ID: 10938

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development