TCR alpha/beta antibody

Catalog No. ABIN7013895

This Mouse Monoclonal antibody specifically detects TCR alpha/beta in FACS, IP, IHC (fro), WB, IHC (p), Func and ICC. It exhibits reactivity toward Rat and Non-Human Primate.

Quick Overview for TCR alpha/beta antibody (ABIN7013895)

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Reactivity: Rat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR alpha/beta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunocytochemistry (ICC)

Clone: R73

Product Details anti-TCR alpha/beta Antibody

Purpose: Anti-Rt TCR alpha/beta Purified Low Endotoxin

Specificity: The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Purification: Purified by protein-A affinity chromatography.

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Rat T blasts and erythrocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Functional application: In vitro activation and in vivo depletion of alpha/beta TCR T cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for TCR alpha/beta

Target: TCR alpha/beta (T Cell Receptor alpha/beta (TCR alpha/beta))

Alternative Name: TCR alpha/beta

Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.,TCRA/B