GRAP2 antibody (PE)

Catalog No. ABIN7013928

This Mouse Monoclonal antibody specifically detects GRAP2 in ICFC. It exhibits reactivity toward Human.

Quick Overview for GRAP2 antibody (PE) (ABIN7013928)

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRAP2 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: UW40

Product Details anti-GRAP2 Antibody (PE)

Purpose: Anti-GRAP2 PE

Specificity: The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: GST-fusion human GRAP2/GADS protein

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Alternative Name: GRAP2

Background: GRB2 related adaptor protein 2,GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.,GADS, GRID, GRAP-2, GrbX

Gene ID: 9402

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway