CD8 alpha antibody

Catalog No. ABIN7013939

This anti-CD8 alpha antibody is a Mouse Monoclonal antibody detecting CD8 alpha in FACS, WB, IP, IHC (fro), IHC (p) and Func. Suitable for Rat.

Quick Overview for CD8 alpha antibody (ABIN7013939)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Clone: OX-8

Product Details anti-CD8 alpha Antibody

Purpose: Anti-Rt CD8a Purified Low Endotoxin

Specificity: The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).

Purification: Purified by protein-A affinity chromatography.

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: High Mw glycoproteins from rat thymocytes

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Functional application: Blocking

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: CD8a molecule,The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-2, Ly-B, Ly-35, Lyt-2

Gene ID: 24930

UniProt: P07725

Pathways: TCR Signaling