PD-L1 antibody (full length)

Catalog No. ABIN7013977

The Mouse Monoclonal anti-PD-L1 antibody is suitable to detect PD-L1 in samples from Human and Non-Human Primate. It has been validated for FACS, Func and IHC (fro).

Quick Overview for PD-L1 antibody (full length) (ABIN7013977)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 29E-2A3

Product Details anti-PD-L1 Antibody

Binding Specificity: full length

Purpose: Anti-Hu CD274 Purified Low Endotoxin

Characteristics: The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.

Purification: Purified by protein-A affinity chromatography.

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Full length human CD274

Isotype: IgG2b, kappa

Application Details

Application Notes: Functional application: Blocking.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background: CD274 Molecule,CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.,B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints