MCAM antibody (PerCP)

Catalog No. ABIN7013999

The PerCP-conjugated Mouse Monoclonal anti-MCAM antibody (Clone P1H12) (ABIN7013999) specifically detects MCAM in FACS. The antibody is reactive with Human, Mouse, Rabbit and Dog samples.

Quick Overview for MCAM antibody (PerCP) (ABIN7013999)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Mouse, Rabbit, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to PerCP

Application: Flow Cytometry (FACS)

Clone: P1H12

Product Details anti-MCAM Antibody (PerCP)

Purpose: Anti-Hu CD146 PerCP

No Cross-Reactivity: Rat

Characteristics: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

Purification: Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: cultured human umbilical cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: Melanoma cell adhesion molecule,CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.,MelCAM, MCAM, MUC18

Gene ID: 4162

UniProt: P43121