Pp1a/Pp2ca/Ppp1ca (AA 281-382) antibody (HRP)

Catalog No. ABIN703670

The Rabbit Polyclonal anti-Pp1a/Pp2ca/Ppp1ca antibody is suitable to detect Pp1a/Pp2ca/Ppp1ca in samples from Mouse. It has been validated for ELISA, IHC (fro) and IHC (p).

Quick Overview for Pp1a/Pp2ca/Ppp1ca (AA 281-382) antibody (HRP) (ABIN703670)

Target: Pp1a/Pp2ca/Ppp1ca

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 281-382

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PPP1CA

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Pp1a/Pp2ca/Ppp1ca

Background:

Synonyms: PP 1A, PP1A, PP2C ALPHA, PP2CA, PPP1CA, Protein Phosphatase 2C Alpha Isoform, Serine threonine protein phosphatase PP1 alpha catalytic subunit, EC 3.1.3.16, MGC15877, MGC1674, MGC9201.

Background: PPP1A is a serine/threonine protein phosphatase catalytic subunit that is essential for regulating cellular stress responses in eukaryotes. It binds to magnesium or manganese ions and exists as a monomer. It is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1A is involved in the regulation long term synaptic plasticity and may play an important role in dephosphorylating substrates such as Ca2+/calmodulin dependent protein kinase II.

Gene ID: 5499

UniProt: P62136