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HTR2A antibody (Extracellular)

HTR2A Reactivity: Rat WB, IHC, IF (cc) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7042875
  • Target See all HTR2A Antibodies
    HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
    Binding Specificity
    • 16
    • 15
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 40-52, Extracellular
    Reactivity
    • 65
    • 53
    • 51
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    Rat
    Host
    • 70
    • 1
    • 1
    Rabbit
    Clonality
    • 72
    Polyclonal
    Conjugate
    • 21
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HTR2A antibody is un-conjugated
    Application
    • 52
    • 27
    • 26
    • 21
    • 12
    • 10
    • 7
    • 6
    • 4
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))
    Purpose
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
    Specificity
    Extracellular, N-terminus
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 9,13 amino acid residues identical
    Characteristics
    Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody is directed against an epitope of the rat 5-HT-2A receptor. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (ABIN7042875, ABIN7045286 and ABIN7045287)) can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is ideal for detecting the receptor in living cells. It has been designed to recognize rat 5-hydroxytryptamine receptor 2A from rat, mouse, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A

    Isotype
    IgG
    Top Product
    Discover our top product HTR2A Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: BLP-SR033

    Blocking Peptide: BLP-SR033

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
    Alternative Name
    HTR2A (HTR2A Products)
    Background
    5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A,5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

    Alternative names: 5HT2A Receptor (HTR2A), 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A
    Gene ID
    29595
    NCBI Accession
    NM_000621
    UniProt
    P14842
    Pathways
    JAK-STAT Signaling, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process
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