HTR2A antibody (Extracellular)
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- Target See all HTR2A Antibodies
- HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
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Binding Specificity
- AA 40-52, Extracellular
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HTR2A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))
- Purpose
- A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
- Specificity
- Extracellular, N-terminus
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Mouse - identical, human - 9,13 amino acid residues identical
- Characteristics
- Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody is directed against an epitope of the rat 5-HT-2A receptor. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (ABIN7042875, ABIN7045286 and ABIN7045287)) can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is ideal for detecting the receptor in living cells. It has been designed to recognize rat 5-hydroxytryptamine receptor 2A from rat, mouse, and human samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A
- Isotype
- IgG
- Top Product
- Discover our top product HTR2A Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200
Application Dilutions Western blot wb: 1:200
- Comment
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Negative Control: BLP-SR033
Blocking Peptide: BLP-SR033
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
- Alternative Name
- HTR2A (HTR2A Products)
- Background
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5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A,5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.
Alternative names: 5HT2A Receptor (HTR2A), 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A - Gene ID
- 29595
- NCBI Accession
- NM_000621
- UniProt
- P14842
- Pathways
- JAK-STAT Signaling, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process
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