HTR2A antibody (AA 296-309)

Catalog No. ABIN1049520

The Goat Polyclonal anti-HTR2A antibody (ABIN1049520) specifically detects HTR2A in IHC, ELISA, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HTR2A antibody (AA 296-309) (ABIN1049520)

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HTR2A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-HTR2A Antibody

Binding Specificity: AA 296-309

Purpose: 5HT2A Receptor (aa296-309)

Sequence: QRSIHREPGS YTGR

Specificity: This antibody is expected to recognize both reported isoforms (NP_000612.1, NP_001159419.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRSIHREPGSYTGR, from the internal region of the protein sequence according to NP_000612.1, NP_001159419.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HTR2A

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Alternative Name: HTR2A

Background: HTR2A, 5-hydroxytryptamine (serotonin) receptor 2A, 5-HT2A, HTR2, 5-HT-2, 5-HT-2A, 5-HT2 receptor, 5-hydroxytryptamine receptor 2A, OTTHUMP00000018378, serotonin 5-HT-2A receptor, serotonin receptor 2A

Gene ID: 3356

NCBI Accession: NP_000612, NP_001159419

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process