HTR7 antibody (Extracellular)

Catalog No. ABIN7042876

This Rabbit Polyclonal antibody specifically detects HTR7 in WB, IHC, IF, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for HTR7 antibody (Extracellular) (ABIN7042876)

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-HTR7 Antibody

Binding Specificity: AA 73-84, Extracellular

Purpose: A Rabbit Polyclonal Antibody to 5HT7 Receptor

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (ABIN7042876, ABIN7045292 and ABIN7045293) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize 5HT7 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7234595)

Blocking Peptide: (ABIN7234595)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HTR7

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Alternative Name: HTR7

Background: 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7,5-HT7 receptor is a G-protein coupled receptor (GPCR) that is activated by Serotonin. Like all other members, 5-HT7 has seven transmembrane domains. In addition, it contains a hydrophobic domain located at its N-terminal end1.The signaling cascades activated by these receptors take part in circadian rhythm, learning and memory, hippocampal signaling and smooth muscle relaxation. It was also shown to have a role in several disorders including Autism, neuropsychiatric disorders, Epilepsy and Alzheimer's disease2,4.5-HT7 is encoded by HTR7 gene and it has three splice variants encoding proteins that differ in the length of their carboxy-terminal ends. The splice variants are differentially expressed in the spleen, kidney, heart, thalamus, hindbrain, hippocampus, cortex, caudate, striatum and cerebellum3.Due to its involvement in central and the peripheral nervous systems, endocrine system and its involvement in various diseases and syndromes, 5-HT7 receptor represents an interesting target for the treatment and prevention of various pathologies. Selective ligands are being studied in order to regulate this receptor's activity4.

Alternative names: 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7

Gene ID: 65032

NCBI Accession: NM_000872

UniProt: P32305

Pathways: JAK-STAT Signaling