AKAP12 antibody (Intracellular)

Catalog No. ABIN7042878

This anti-AKAP12 antibody is a Rabbit Polyclonal antibody detecting AKAP12 in WB, IHC and IF. Suitable for Mouse.

Quick Overview for AKAP12 antibody (Intracellular) (ABIN7042878)

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-AKAP12 Antibody

Binding Specificity: AA 1639-1652, Intracellular

Purpose: A Rabbit Polyclonal Antibody to AKAP12

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-AKAP12 Antibody (ABIN7042878, ABIN7045178 and ABIN7045179) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gravin from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GNLTKESPDTNGPK, corresponding to amino acid residues 1639-1652 of mouse AKAP12

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234603)

Blocking Peptide: (ABIN7234603)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP12

Background: A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS,The A-kinase anchoring protein 12 (AKAP12) belongs to a family of scaffold proteins, which anchor signaling proteins such as PKA in a spatiotemporal manner4. AKAPs generally bind PKA at their N-terminus, while the sequence at their C-terminus determines their spatial location4. To date, more than 50 AKAPs have been identified and perceived to integrate signaling pathways by anchoring multi-protein complexes1.AKAP12 integrates diverse signaling pathways such as PKA, PKC, and β2-adrenergic receptor7,8. Similar to other members of this protein-family, AKAP12 contains a PKA binding domain. In addition, AKAP12 also contains a docking site for PKC2,6 and a myristoyl acid that sequesters it to the plasma membrane7.AKAP12 is widely expressed in a variety of tissues with the highest expression in male and female reproductive organs, skeletal muscle, kidneys, and urinary tract5.One of the most characterized activities of AKAP12 is regulation of the cell cycle3. AKAP12 controls two fundamental cell processes such as the G1/S transition and cytokinesis. In that being said, studies in NIH-3T3 fibroblasts, showed that ectopic expression of AKAP12 reduced cyclin D1 levels and the phosphorylation of its downstream target Rb, and consequently attenuated cell-cycle progression3. Furthermore, in HeLa cells, knockdown of AKAP12 resulted in profound changes in cell morphology, which indicates the inability of cells to divide3.Given that AKAP12 plays a pivotal role in cell cycle regulation, it is not surprising that in some cases it is involved in cancer malignancies. It is now clear that AKAP12 is a key driver of gastrointestinal cancers such as colorectal cancer and hepatocellular carcinomas8.

Alternative names: AKAP12, A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS

Gene ID: 83397

NCBI Accession: NM_001370346

UniProt: Q9WTQ5

Pathways: cAMP Metabolic Process