ADORA1 antibody (AA 213-229)

Catalog No. ABIN7042882

Product Details anti-ADORA1 Antibody

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Binding Specificity: AA 213-229

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Adenosine A1 Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat,bovine,canis,pig - identical

Characteristics: Anti-Adenosine A1 Receptor Antibody is directed against human adenosine A1 receptor. Anti-Adenosine A1 Receptor Antibody (ABIN7042882, ABIN7043894 and ABIN7043895)) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize adenosine A1R from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-AR006

Blocking Peptide: BLP-AR006

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1 (ADORA1 Products)

Background: ADORA1, A1AR, Adenosine A1R,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10

Alternative names: Adenosine A1 Receptor, ADORA1, A1AR, Adenosine A1R

Gene ID: 134

NCBI Accession: NM_000674

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane