Adenosine A2a Receptor antibody (Extracellular)

Catalog No. ABIN7042886

The Rabbit Polyclonal anti-Adenosine A2a Receptor antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect Adenosine A2a Receptor in samples from Mouse.

Quick Overview for Adenosine A2a Receptor antibody (Extracellular) (ABIN7042886)

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Adenosine A2a Receptor Antibody

Binding Specificity: AA 143-156, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): The antibody will not recognize adenosine A2A receptor from human samples.

Predicted Reactivity: 14 amino acid residues identical, rat - 12,Mouse - 13

Characteristics: Anti-Adenosine A2A Receptor (extracellular) Antibody (ABIN7042886, ABIN7043898 and ABIN7043899) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize A2aR from mouse and rat samples. The antibody will not recognize the receptor from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A2A receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234630)

Blocking Peptide: (ABIN7234630)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A

Background: ADORA2A, A2AAR, Adenosine A2aR,Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins. The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

Alternative names: Adenosine A2A Receptor, ADORA2A, A2AAR, Adenosine A2aR

Gene ID: 11540

NCBI Accession: NM_000675

UniProt: Q60613

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints