ADCYAP1R1 antibody (Intracellular)

Catalog No. ABIN7042894

This Rabbit Polyclonal antibody specifically detects ADCYAP1R1 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for ADCYAP1R1 antibody (Intracellular) (ABIN7042894)

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADCYAP1R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-ADCYAP1R1 Antibody

Binding Specificity: AA 474-487, Intracellular

Purpose: A Rabbit Polyclonal Antibody to PACAP Type I Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,14 amino acid residues identical

Characteristics: Anti-PACAP Receptor 1 (PAC1) Antibody is directed against an epitope of the rat PACAP type I receptor. Anti-PACAP Receptor 1 (PAC1) Antibody (ABIN7042894, ABIN7045333 and ABIN7045334) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Comment:

Cited Application: IHC

Negative Control: (ABIN7234650)

Blocking Peptide: (ABIN7234650)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADCYAP1R1

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Alternative Name: ADCYAP1R1

Background: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1,Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways1. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition2.PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages3.PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety4.PAC1 receptor null mice demonstrate reduced anxiety-like behavior5. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology1.

Alternative names: PACAP Receptor 1 (PAC1), PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Gene ID: 24167

NCBI Accession: NM_001118

UniProt: P32215

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process