ADCYAP1R1 antibody (N-Term)

Catalog No. ABIN1048297

Product Details anti-ADCYAP1R1 Antibody

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Binding Specificity: N-Term

Reactivity: Human, Monkey, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADCYAP1R1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ADCYAP1R1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%) Dog, Pig (94%) Mouse, Rat, Elephant, Panda (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-terminal extracellular domain of human ADCYAP1R1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%), Dog, Pig (94%), Mouse, Rat, Elephant, Panda (89%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Usage: Antigen retrieval requires Proteinase K treatment.

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ADCYAP1R1

Target: ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))

Alternative Name: ADCYAP1R1 / PAC1 Receptor (ADCYAP1R1 Products)

Synonyms: PAC1 antibody, PAC1R antibody, PACAPR antibody, PACAPRI antibody, 2900024I10Rik antibody, AI846590 antibody, PACAP1-R antibody, pac1 antibody, pacapr antibody, pacapri antibody, PAC1-R antibody, PACAP-R1 antibody, PACAP-R1A antibody, PACAPR1 antibody, PACAPR1A antibody, adcyap1r1 antibody, adcyap1r1-A antibody, ADCYAP1R1 antibody, ADCYAP receptor type I antibody, adenylate cyclase activating polypeptide 1 receptor 1 antibody, adenylate cyclase activating polypeptide 1 (pituitary) receptor type I antibody, ADCYAP receptor type 1 antibody, adenylate cyclase activating polypeptide 1a (pituitary) receptor type I antibody, adenylate cyclase activating polypeptide 1 (pituitary) receptor type I S homeolog antibody, ADCYAP1R1 antibody, Adcyap1r1 antibody, adcyap1r1 antibody, adcyap1r1a antibody, adcyap1r1.S antibody

Background: Name/Gene ID: ADCYAP1R1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR

Synonyms: ADCYAP1R1, PAC1, Pac1 receptor, Pac1 receptors, PAC1-R, PACAP receptor 1, Pacap specific receptor, PACAP-R-1, PACAP/VIP receptors, PACAP1 receptor, PACAP Receptor, Pacap receptor type 1, Pacap type 1 receptor, PACAP type I receptor, PACAPR, PAC1R, PACAP-R1, PACAPRI, Pvr1, Pacap receptor type i

Gene ID: 117

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process