ADRB1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886061

The Rabbit Polyclonal anti-ADRB1 antibody (ABIN7886061) specifically detects ADRB1 in WB, FACS, IHC, IF and LCI. The antibody is reactive with Rat samples.

Quick Overview for ADRB1 antibody (2nd Extracellular Loop) (ABIN7886061)

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-ADRB1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 200-214

Purpose: A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,Mouse,human - 14,15 amino acid residues identical

Characteristics: Anti-β1-Adrenergic Receptor (extracellular) Antibody (ABIN7042902, ABIN7043924 and ABIN7043925) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RAESDEARR(S)YNDPK, corresponding to amino acid residues 200-214 of rat ADRB1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234672)

Blocking Peptide: (ABIN7234672)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ADRB1

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Alternative Name: ADRB1

Background:

Synonyms: Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Description: β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2.β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4.β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.

Gene ID: 64508

NCBI Accession: NM_000684

UniProt: P18090

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation