MAS1 antibody (AA 212-224)

Catalog No. ABIN7042920

Product Details anti-MAS1 Antibody

Target: MAS1 (MAS1 Oncogene (MAS1))

Binding Specificity: AA 212-224

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Angiotensin-(1-7) Mas Receptor Antibody (ABIN7042920, ABIN7043904 and ABIN7043905)) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC|IFC

Negative Control: BLP-AR013

Blocking Peptide: BLP-AR013

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MAS1

Target: MAS1 (MAS1 Oncogene (MAS1))

Alternative Name: MAS1 (MAS1 Products)

Background: Proto-oncogene Mas, MAS1,The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide Angiotensin-(1-7).1Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

Alternative names: Angiotensin-(1-7) Mas Receptor, Proto-oncogene Mas, MAS1

Gene ID: 25153

NCBI Accession: NM_002377

UniProt: P12526

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process