Anoctamin 5 antibody (AA 532-544)

Catalog No. ABIN7042929

Product Details anti-Anoctamin 5 Antibody

Target: Anoctamin 5 (ANO5)

Binding Specificity: AA 532-544

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 5 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Anoctamin-5 Channel

Specificity: 2nd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-Anoctamin-5 Antibody is directed against an epitope of mouse ANO5. Anti-Anoctamin-5 Antibody (ABIN7042929, ABIN7044135 and ABIN7044136)) can be used in western blot analysis. It has been designed to recognize ANO5 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CL015

Blocking Peptide: BLP-CL015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Anoctamin 5

Target: Anoctamin 5 (ANO5)

Alternative Name: ANO5 (ANO5 Products)

Background: ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L,Anoctamins, also known as TMEM16, is a family of ten proteins involved in a variety of functions including phospholipid scrambling, ion transport and the regulation of other membrane proteins. ANO1 (Anoctamin-1), ANO2 and ANO5 function as Ca2+-activated Cl- channels (CaCCs) and play an important role in calcium dependent chloride secretion. All members of the Anoctamin family present high structural homology and consist of eight transmembrane domains and cytosolic N- and C-termini1.The ANO5 gene is widely distributed and expressed in bone, skeletal muscle, cardiac muscle, brain, lung and kidney and also in the mucosal layer of the GI tract and the skeletal muscle layer of the esophagus of mice2.In phylogenetic analysis ANO5 was found to be a close relative of ANO6. ANO6 has an association with phospholipid scramblase activity. Under both physiological and pathological conditions scramblase dissipates the normal asymmetry of the cell membrane by catalyzing the rapid externalization of phospholipids on the cell membrane and thus promoting apoptosis3.Recessive mutations in the ANO5 gene were found to be linked to limb-girdle muscular dystrophies type 2L (LGMD2L) and non-dysferlin Miyoshi myopathy4. LGMD2L is characterized by proximal weakness, asymmetric quadriceps femoris and atrophy of the biceps brachii muscle5.Mutations in the gene are also linked to the Gnathodiaphyseal dysplasia disease, an unusual generalized autosomal dominant disease. Patients with this syndrome show sclerosis of tubular bones, lesions of jawbones, an increased sensitivity for jaw infection and osteomyeletis and fragile bones6.

Alternative names: Anoctamin-5, ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L

Gene ID: 233246

NCBI Accession: NM_213599

UniProt: Q75UR0