AQP6 antibody (Intracellular)

Catalog No. ABIN7042942

Product Details anti-AQP6 Antibody

Target: AQP6 (Aquaporin 6, Kidney Specific (AQP6))

Binding Specificity: AA 259-276, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 6 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - 15,18 amino acid residues identical

Characteristics: Anti-Aquaporin 6 Antibody is directed against an epitope of rat AQP6. Anti-Aquaporin 6 Antibody (ABIN7042942 and ABIN7045202)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP6 from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EPQKKESQTNSEDTEVSV corresponding to amino acid residues 259-276 of rat AQP6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-QP006

Blocking Peptide: BLP-QP006

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AQP6

Target: AQP6 (Aquaporin 6, Kidney Specific (AQP6))

Alternative Name: AQP6 (AQP6 Products)

Background: AQP6, AQP2L, Kidney-specific aquaporin,It has been long known that water must cross biological membranes by means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question was answered. The importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels. Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini.Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney, particularly in intracellular vesicles in collecting duct intercalated cells. Unlike the other aquaporins, AQP6 was found to allow permeation of anions following activation with acidic pH or Hg2+ ions. Increased expression of AQP6 was shown in models of chronic alkalosis and lithium-induced nephrogenic diabetes insipidus.

Alternative names: Aquaporin 6, AQP6, AQP2L, Kidney-specific aquaporin

Gene ID: 29170

UniProt: Q9WTY0