Aquaporin 7 antibody (Intracellular)

Catalog No. ABIN7042943

Product Details anti-Aquaporin 7 Antibody

Target: Aquaporin 7 (AQP7)

Binding Specificity: AA 7-24, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 7 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical,Mouse - 15, human - 11

Characteristics: Anti-Aquaporin 7 Antibody (ABIN7042943, ABIN7045203 and ABIN7045204)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize AQP-7 from mouse and rat samples, and may recognize the channel from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ENIQSVLQKTWVREFLAE, corresponding to amino acid residues 7-24 of rat AQP7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:500

Comment:

Cited Application: IHC

Negative Control: BLP-QP007

Blocking Peptide: BLP-QP007

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 7

Target: Aquaporin 7 (AQP7)

Alternative Name: AQP7 (AQP7 Products)

Background: AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose,Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.AQP-7 is expressed in ovary, testis, kidney and adipose tissue.The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.

Alternative names: Aquaporin 7, AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose

Gene ID: 29171

NCBI Accession: NM_001170

UniProt: P56403