Aquaporin 7 antibody (Intracellular)

Catalog No. ABIN7042943

Product Details anti-Aquaporin 7 Antibody

Target: Aquaporin 7 (AQP7)

Binding Specificity: AA 7-24, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 7 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical,Mouse - 15, human - 11

Characteristics: Anti-Aquaporin 7 Antibody (ABIN7042943, ABIN7045203 and ABIN7045204) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize from mouse and rat samples, and may recognize the channel from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ENIQSVLQKTWVREFLAE, corresponding to amino acid residues 7-24 of rat AQP7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:500

Comment:

Cited Application: IHC

Negative Control: (ABIN7234775)

Blocking Peptide: (ABIN7234775)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 7

Target: Aquaporin 7 (AQP7)

Alternative Name: AQP7 (AQP7 Products)

Background: AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose,Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.AQP-7 is expressed in ovary, testis, kidney and adipose tissue.The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.

Alternative names: Aquaporin 7, AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose

Gene ID: 29171

NCBI Accession: NM_001170

UniProt: P56403