AVPR2 antibody (Intracellular)

Catalog No. ABIN7042948

This Rabbit Polyclonal antibody specifically detects AVPR2 in WB. It exhibits reactivity toward Rat.

Quick Overview for AVPR2 antibody (Intracellular) (ABIN7042948)

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AVPR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AVPR2 Antibody

Binding Specificity: AA 345-358, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,14 amino acid residues identical

Characteristics: Anti-Vasopressin V2 Receptor (AVPR2) Antibody is directed against an epitope of rat Arginine vasopressin receptor 2. Anti-Vasopressin V2 Receptor (AVPR2) Antibody (ABIN7042948, ABIN7045339 and ABIN7045340) can be used in western blot analysis. It has been designed to recognize V2R from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RHTTHSLGPQDESC, corresponding to amino acid residues 345-358 of rat AVPR2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7234794)

Blocking Peptide: (ABIN7234794)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AVPR2

Target: AVPR2 (Arginine Vasopressin Receptor 2 (AVPR2))

Alternative Name: AVPR2

Background: V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus,Vasopressin V2 receptor (V2R), or arginine vasopressin receptor 2 (AVPR2), is a G-protein-coupled receptor (GPCR), coupled to the stimulatory heterotrimeric GTP-binding protein, GS, that elevates intracellular cAMP through activation of adenylate cyclase1-3. Like all members, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.AVPR2 is expressed primarily in renal collecting ducts and loop of Henle of the kidney, but also in vascular smooth muscle, where it induces vasodilation. Its ligand is the anti-diuretic hormone (ADH), aka vasopressin, a hormone that originates from the posterior pituitary gland that has key roles in water homeostasis and in hypotension5.Vasopressin triggers a variety of receptors - V1R, V2R, V3R, oxytocin and purinergic receptors, which regulate the physiological water-balance. High levels of inflammatory factors in the blood, such as the case in sepsis, are known to down-regulate the vasopressin receptors3.Mutations in V2R that prevent the receptor from reaching and anchoring to the cell surface are responsible are detected in diabetes insipidus and heart failure4,6,7 just to name a few. Interestingly, it was found that female rats possess more V2R mRNA than male rats leading to increased urine osmolality and decreased urine volume in females compared with males - a finding which explains that clinical data according to which females are more prone to suffer from hyponatremia4. A recent study also showed that vasopressin interacts with V2R in sperm-cell membranes to enhance ion transport, over expression of the hormone reduced sperm motility, hence V2R also has an important part in male fertility8.

Alternative names: Vasopressin V2 Receptor (AVPR2), V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus

Gene ID: 25108

NCBI Accession: NM_000054

UniProt: Q00788

Pathways: cAMP Metabolic Process