Astrotactin 1 (ASTN1) (AA 1068-1080), (Extracellular) antibody
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- Target See all Astrotactin 1 (ASTN1) Antibodies
- Astrotactin 1 (ASTN1)
- Binding Specificity
- AA 1068-1080, Extracellular
- Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Purpose
- A Rabbit Polyclonal Antibody to Astrotactin-1
- Specificity
- Extracellular, C-terminus
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Rat,human - identical
- Characteristics
- Anti-ASTN1 (extracellular) Antibody (ABIN7042949, ABIN7044732 and ABIN7044733)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Astrotactin-1 from rat, mouse, and human samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1
- Isotype
- IgG
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
- Comment
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Negative Control: BLP-NR151
Blocking Peptide: BLP-NR151
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 0.2 mL double distilled water (DDW).
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- Astrotactin 1 (ASTN1)
- Alternative Name
- ASTN1 (ASTN1 Products)
- Background
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Astrotactin-1,Astrotactin 1 (ASTN-1) is an integral membrane perforin-like protein known to play a critical role in neurodevelopment. The protein is responsible for the formation of neuron-glial fiber contacts in the cerebellum where it plays an important role in neuronal migration1,2.Astrotactin 1 is also expressed in post-mitotic neuronal precursors of the cerebellum, hippocampus, cerebrum and olfactory bulb. The protein demonstrates an active role in the establishment of laminar structures1.Astrotactin 1 structure reveals a large extracellular C-terminal domain that is complemented by an N-terminal cytosolic domain separated by two transmembrane α-helices1.Studies have found that genetic variations associated with Astrotactin 1 are connected to various neurodevelopmental disorders and other neurological pathologies including Alzheimer's disease1. In addition, ASTN1 null mice exhibit slow migration rates of cerebellar granule cells, smaller cerebellar size and reduced glial neuron binding2 and poorer balance and coordination than wild-type1. Conversely, increased levels of Astrotactin 1 are detected in rat brain following hippocampal injury, linking the protein to repair processes.
Alternative names: ASTN1, Astrotactin-1 - Gene ID
- 11899
- NCBI Accession
- NM_004319
- UniProt
- Q61137
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