Astrotactin 1 (ASTN1) (AA 1068-1080), (C-Term), (Extracellular) antibody

Catalog No. ABIN7886677

The Rabbit Polyclonal anti-Astrotactin 1 antibody is suitable to detect Astrotactin 1 in samples from Mouse. It has been validated for WB, IF and IHC.

Quick Overview for Astrotactin 1 (ASTN1) (AA 1068-1080), (C-Term), (Extracellular) antibody (ABIN7886677)

Target: Astrotactin 1 (ASTN1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 1068-1080, C-Term, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Astrotactin-1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-ASTN1 (extracellular) Antibody (ABIN7042949, ABIN7044732 and ABIN7044733) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Astrotactin-1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RQEKVTDRMDHSK, corresponding to amino acid residues 1068-1080 of mouse Astrotactin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234798)

Blocking Peptide: (ABIN7234798)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details

Target: Astrotactin 1 (ASTN1)

Alternative Name: ASTN1

Background:

Synonyms: Astrotactin-1

Description: Astrotactin 1 (ASTN-1) is an integral membrane perforin-like protein known to play a critical role in neurodevelopment. The protein is responsible for the formation of neuron-glial fiber contacts in the cerebellum where it plays an important role in neuronal migration1,2.Astrotactin 1 is also expressed in post-mitotic neuronal precursors of the cerebellum, hippocampus, cerebrum and olfactory bulb. The protein demonstrates an active role in the establishment of laminar structures1.Astrotactin 1 structure reveals a large extracellular C-terminal domain that is complemented by an N-terminal cytosolic domain separated by two transmembrane α-helices1.Studies have found that genetic variations associated with Astrotactin 1 are connected to various neurodevelopmental disorders and other neurological pathologies including Alzheimer's disease1. In addition, ASTN1 null mice exhibit slow migration rates of cerebellar granule cells, smaller cerebellar size and reduced glial neuron binding2 and poorer balance and coordination than wild-type1. Conversely, increased levels of Astrotactin 1 are detected in rat brain following hippocampal injury, linking the protein to repair processes.

Gene ID: 11899

NCBI Accession: NM_004319

UniProt: Q61137