ATP6AP2 antibody (Extracellular)

Catalog No. ABIN7042960

Product Details anti-ATP6AP2 Antibody

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Binding Specificity: AA 200-214, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6AP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to Renin Receptor

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).

Predicted Reactivity: Rat,human - identical

Characteristics: A Rabbit Polyclonal Antibody to Renin Receptor

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234840)

Blocking Peptide: (ABIN7234840)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: ATP6AP2 (ATP6AP2 Products)

Background: Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2,The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin. Renin is an aspartyl protease that cleaves angiotensinogen into angiotensin I, the rate-limiting reaction in the cascade generating angiotensin, a peptide hormone that is involved in blood pressure regulation. Renin binding by (P)RR affects important biological systems and pathways that are involved in cell cycle regulation, autophagy, acid-base balance, energy metabolism, embryonic development, T cell homeostasis, water balance, blood pressure regulation, cardiac remodeling and maintenance of podocyte structure1.In addition, (P)RR has been reported to contribute to the pathogenesis of diseases such as fibrosis, hypertension, pre-eclampsia, diabetic microangiopathy, acute kidney injury, cardiovascular disease, cancer and obesity1.The receptor is widely distributed in organs throughout the body, including the kidneys, heart, brain, eyes, placenta, and the immune system1.

Alternative names: Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2

Gene ID: 70495

NCBI Accession: NM_005765

UniProt: Q9CYN9

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones